No abstract

We isolated a strain coded Esc2AmT during a study focused on the microbial diversity of adult specimens of the bark beetle Hylesinus fraxini. Its 16S rRNA gene sequence had 99.4 % similarity with respect to its closest relative, Pseudomonas rhizosphaerae IH5T . The analysis of partial sequences of the housekeeping genes rpoB, rpoD and gyrB confirmed that strain Esc2AmT formed a cluster with P. rhizosphaerae IH5 T clearly separated from the remaining species of the genus Pseudomonas. Strain Esc2Am T had polar flagella and could grow at temperatures from 4 8C to 30 8C. The respiratory quinone was Q9 and the main fatty acids were C 16 : 0 , C 18 : 1 v7c and/or C 18 : 1 v6c in summed feature 8 and C 16 : 1 v7c and/or C 16 : 1 v6c in summed feature 3. DNA-DNA hybridization results showed 51 % relatedness with respect to P. rhizosphaerae IH5 T . Oxidase, catalase and urease-positive, the arginine dihydrolase system was present but nitrate reduction and b-galactosidase production were negative. Aesculin hydrolysis was positive. Based on the results from the genotypic, phenotypic and chemotaxonomic analyses, we propose the classification of strain Esc2AmT as representing a novel species of the genus Pseudomonas, for which we propose the name Pseudomonas coleopterorum sp. nov. The type strain is Esc2Am T (5LMG 28558 T 5CECT 8695 T ).