No abstract

No abstract

of whole genome (28.7 million CpG sites). The average DNAme level was 15%. Aneuploid embryos showed significantly lower DNAme levels compared to euploid embryos (p<0.0001). Increased patient age was correlated with elevated DNAme levels in blastocysts (p¼0.04). Blastocysts cryopreserved on day 6 had significantly lower DNAme compared to those that were cryopreserved on day 5 (p¼0.001). Whole chromosomal aneuploidy predicted by calculating the fraction of read count from each chromosome showed 100% consistence with previous diagnosis. The chromosomes involved in monosomy embryos (-4,-13,-16, and -18) showed reduced methylation rates compared to the other chromosomes.CONCLUSIONS: DNAme levels detected by RRBS in trophoectoderm biopsies from human blastocysts is associated with ploidy status, maternal age, and embryo growth characteristics, This novel tool could may provide a foundation for the development of epigenetic biomarkers of reproductive competence.

No abstract

В обзоре представлены данные литературы за последние 10-15 лет, касающиеся изучения цитоморфологических особенностей ооцитов человека и их влияния на частоту имплантации и наступление клинической беременности. Описаны новые подходы к проведению стимуляции овариальной функции яичников.