Eurycoma longifolia Jack is well known among the communities in Southeast Asia because of its aphrodisiac properties and its effectiveness as the cytotoxic, anti-malarial, anti-ulcer, anti-tumor promoting and anti-parasitic agent. Micropropagation through direct plant regeneration from in vivo shoot tip explants was carried out. The highest regeneration percentage (90%) and multiple shoots formation were obtained with the basal Murashige and Skoog (MS) medium supplemented with 5.0 mg l Ϫ1 kinetin. Roots were induced after 14 days of culture in the basal MS medium supplemented with 0.5 mg l Ϫ1 of indole-3-butyric acid. Plantlets regenerated from shoot tip explants survived well with no morphological differences from parent plants after two months of transplantation to soil.

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One of the most important preliminary investigation of callus attributes is the growth characteristics. Most often than not, callus growth curve is sigmoidal in characteristics. In this work, we model callus growth from the seedling of Jatropha curcas L. according to the modified Gompertz model from published literature to acquire essential growth constants. These growth constants can be obtained with better precision using model such as the modified Gompertz. Parameters obtained from the fitting exercise were maximum callus growth rate (m), lag time () and maximal callus production (Ymax) of 0.193 d-1, 2.91 days and 0.38 g callus/25 mL culture, respectively. Growth parameter constants extracted from the modelling exercise will be helpful for additional secondary modelling implicating the consequence of media conditions as well as other factors on the growth of callus from this plant.

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