HPLC-mass spectrometry is a useful method for the highly sensitive and simultaneous detection of histamine and N-methylhistamine. Histamine is present in virtually all organs, not only in those traditionally associated with histamine-mediated disease. Highest concentrations are found in stomach, lymph node, and thymus; medium concentrations in heart, spleen, and kidney; and lowest concentrations detected in intestine, brain, liver, and lung.

D e p a r m t of P e d i a t r i c s .Can o l e a t e and BOHB s u b s t i t u t e f o r glucose as c e r e b r a l energy f u e l s a f t e r hypoxia? 0leate-U-14C o r BOHB-3-14C were added t o 7 days o l d c u l t u r e s after 4 hours preincubation a t 0 o r 4 nM g l u c under n o m i a o r hypoxia. During 3 hours of incubation of a l l c u l t u r e s under norm i a . 14CO7 was collected. Definiriq 032-production a t 4 nM q l u c a s lob%, ~05-production a t 0 vs. 4 qi& was 1562404 in no&-x i a and 162224% a f t e r hypaxia f o r BOHB, t h e correspadir,g values f o r CO2-production f r a n o l e a t e were 218266 and 261+503 (mean+ 1 SD, n=15-25, p 0 v s 4 nM gluc