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Ten new ortho, meta and para substituted derivatives of 2-benzylthio-6-aminouracils have been prepared. Electron Impact (EI) induced mass spectral fragmentation of these compounds was investigated. Fragmentation pathways are proposed on the basis of accurate mass and metastable transition measurements. The correlation between the intensities of the M +. and the selected fragment ions of these compounds is discussed. The data obtained create the basis for dinstinguishing isomers. The 1 H and 13 C NMR spectra of these compounds were assigned unambiguously using a combination of heteronuclear (HETCOR) spectra the chemical shifts. The data derived from these spectra can be used to differentiate the isomers.

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