Measurement of colloidal particles size was performed on a NICOMP 380 ZLS. Pharmacokinetics. Radiometry of the organs was carried out on γ-spectrometer. Measurement of the sizes of radioactive particles of 99mTc-nanotech showed that average dynamic diameter of RFP particle ranged from 16.7 to 24.5 nm. 99mTc-nanotech received by the blood is actively trapped by liver and spleen. Inguinal lymph node in all animals was clearly visualized during lymphoscintigraphy on the 15th minute. Maximum accumulation of 99mTc-nanotech was observed in 3 hours. Experimental study of radiopharmaceutical 99mTc-nanotech showed that the nanocolloid can be used for lymphoscintigraphy and visualization of “sentinel” nodes.

Studying the possibility of using the radiopharmaceutical to identify sentinel lymph nodes were carried out on male rats. The preparation was injected subcutaneously in the I interfinger space of the right forelimb. Radiometry of rats’ organs showed that the studied preparation was actively accumulated in the axillary lymph node after subcutaneous injection. The experimental study of the pharmacokinetics of radiopharmaceutical based on gamma-oxide aluminum labeled with 99mTc showed that the analyzed RPP can be successfully used for lymphoscintigraphy and visualization of sentinel node starting with the 15th minute and up to the 24th hour after subcutaneous injection.

The aim of the investigation was to assess the allergizing properties of a new nanocolloidal preparation based on aluminum gamma-oxide labeled by technetium-99m for scintigraphic and intraoperative detection of sentinel lymphatic nodes in experiment.Materials and Methods. A series of experiments was performed on 80 guinea pigs, 30 Balb/c mice, 40 CBA/CaLac mice and 70 outbred mice of both genders. To study allergenicity various doses of preparation were administered subcutaneously, intramuscularly, intravenously, intradermally as a single injection or by a course. Allergizing properties of the radiopharmaceutical were investigated according to the "Guidelines on the assessment of allergizing properties of drugs".Results. Administration of Nanocolloid, Tc-Al 2 o 3 radiopharmaceutical did not result in the intensification of anaphylaxis systemic reaction in the animals of the examined groups relative to the control. A single injection did not cause inflammatory reaction to Concanavalin A in the examined animals. When guinea pigs were immunized by the preparation, no delayed allergic reaction was revealed. Studying sensitizing effect of the preparation by 20 skin applications no redness or edema of the skin at the site of application was observed.Conclusion. Nanocolloid, 99mTc-Al 2 o 3 radiopharmaceutical in the studied doses and under the selected experimental conditions does not possess allergizing properties.

We study the possibilities of radionuclide methods of research for identification of sentinel lymph nodes (SLN) in gastric cancer. As a result of the intraoperative radiometric research the SLN were identified in all 48 patients. The study of distant lymph nodes reveals the presence of a metastatic lesion of SLN in 27 patients (56.2 %). In 21 patients (43.8 %) there was no metastatic lesion of SLN. Intraoperative radiometric indication allows to determine the "sentinel" lymph nodes with a sensitivity of 100% and a specificity of 100%. The study of "sentinel" lymph nodes is indicated for patients with depth of invasion of T1-T2 and tumor size of less than 3 cm.

To study the diagnostic capabilities of planar breast scintigraphy and single photon emission computed tomography (SPECT) with 99mTc-MIBI in visualizing of breast tumors, 61 patients with a diagnosis of breast cancer were included in the study. The results showed that sensitivity of the planar mode in identifying primary breast tumors was 46%. The sensitivity of SPECT in detecting primary tumor was 93.4%. The sensitivity of the planar mode in visualization of nodal metastases was 44.8%, against 93.1% in SPECT. This study showed that SPECT has a high diagnostic efficiency in regard of visualization of small sized tumors, multicenter tumor growth, identifying space-occupying lesions on the background of modified breast tissue.