We elucidated the mechanisms of action of two n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in Jurkat T-cells. Both DHA and EPA were principally incorporated into phospholipids in the following order: phosphatidylcholine Ͻ phosphatidylethanolamine Ͻ phosphatidylinositol/phosphatidylserine. Furthermore, two isoforms of phospholipase A 2 (i.e., calcium-dependent and calcium-independent) were implicated in the release of DHA and EPA, respectively, during activation of these cells.

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