Knowledge of the persistence of AVs in humans is crucial to our understanding of the nature of AV infection (chronic or acute) and the role of AV in the host. We therefore investigated the dynamics of anellovirus infection in two healthy people followed up for 30 years. Our findings suggest that the human blood anellovirus virome (anellome) remains stable and personal for decades.

Highlights d Cytotoxic T cells form a synapse-like structure during transcellular migration d This diapedesis synapse is enhanced by fractalkine and results in ICAM-1 clustering d This clustering induces the endothelial secretory machinery by recruiting SNAP23 d SNAP23 mediates the synaptic release of chemokines to trigger transcellular migration