Background: Annexin-A1 is an important regulator of VEGF-mediated endothelial cell migration and angiogenesis. Results: We found that miR-196a targets Annexin-A1 to inhibit VEGF-mediated cell migration and angiogenesis. Moreover, VEGF decreases miR-196a and increases Annexin-A1. Conclusion: VEGF-induced decrease of miR-196a expression may participate to the angiogenic switch. Significance: These results bring important new insights in understanding the mechanisms underlying angiogenesis-associated pathologies.