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Clonal analysis represents the gold standard for assaying the differentiation potential of stem cells in vitro. Here we describe Elektra™, a system that permits efficient spotting of single cell clones in a coculture paradigm. Joining their expertise, Evotec Technologies and LIFE & BRAIN showed that neural precursors derived from human embryonic stem (ES) cells survive single cell spotting and continue to generate individual clones. This technology should be particularly useful for the high-throughput clonal analyses increasingly required for human stem cell applications.

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Laser-induced tissue heating and coagulation is a common technique in minimally invasive surgery to achieve localized thermal tissue damage. Precision and efficiency of treatment could be improved markedly by noninvasive spatio-temporal temperature monitoring. A method for two-dimensional mapping of tissue temperature as well as tissue structural changes has been investigated in vitro. The algorithms are based on the temperature dependence of speed of sound and a combination of additional acoustical parameters in order to consider the expansion of the heated volume, creation of gas bubbles, and carbonization. An approach for motion compensation, as it is necessary for clinical application, will be described in short.