Background: Remnants of dental calculus, contaminated cementum, and subgingival plaque act  as  a  physical  barrier  between  periodontal  tissues  and  root  surfaces,  which  inhibits  the formation of new attachment. Treatment of denuded root surface using various chemicals and antimicrobial agents with scaling and root planing helps in active periodontal regeneration. Aim: The aim of this study was to evaluate and compare the demineralizing effects of Triphala and 1% Tetracycline HCl gel on root conditioning using a Scanning Electron Microscope. Settings and  design:  A  total  of  50  hemi-sectioned  root  samples  were  obtained  from  25 extracted,  and  root  planed  samples.  These  were  divided  into  two  groups,  Group  1:  1% Tetracycline HCl gel and Group 2: Triphala. Materials and method: The two root conditioning agents were applied with cotton pellets on the sectioned roots. The samples were then examined under the scanning electron microscope. Statistical analysis used: Data obtained was compiled on a MS Office Excel Sheet. Inter group comparison was done using Mann Whitney U test. Comparison of frequencies of categories of variables with groups was done using Chi Square Test. Results: The root conditioning agents used in this study were found to be equally effective in removing the smear layer. 

BACKGROUND: Periodontal disease is associated with colonization of specific bacteria in the subgingival area. Widespread use of antimicrobials has resulted in emergence of various side effects and drug resistance. Phytomedicine has been used in many clinical conditions and it appears to be a suitable alternative to manage conditions affecting the oral cavity. AIM: Present study aims to assess the in-vitro antimicrobial efficacy of Tulsi extract and Curcumin extract on Periodontal pathogens. MATERIALS & METHODS: Ethanolic extract of 100% w/v of Tulsi and Curcumin was prepared by cold extraction technique. Prepared extracts were further diluted to obtain solutions of 5 different concentrations to be tested (0.5%, 1%, 2% and 5%). Obtained solutions and controls (0.2% Chlorhexidine and Distilled water) were subjected to the microbiological investigation against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Agar well diffusion method was employed to determine the concentration at which Tulsi and Curcumin gave an inhibition zone, similar to Chlorhexidine. Data was analysed using one‑way analysis of variance and post‑hoc test for inter-group and intra‑group comparisons. RESULTS: At 10% concentration both Tulsi and Curcumin extract have demonstrated comparable antimicrobial activity against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

The aim of the study was to compare and evaluate the effect of diode laser as an adjunct to scaling and root planing and scaling and root planing alone on clinical parameters and Gingival Crevicular Fluid (GCF) interlukin-10 level in chronic periodontitis. Ten chronic periodontitis patients were selected. As it was a split mouth study, two sites with deepest pocket one in each quadrant were randomly assigned for scaling and root planing (SRP) and SRP+ diode laser treatment. Plaque index, gingival index, probing depth, and clinical attachment level were measured at baseline and at 3months after treatment. The GCF levels of interleukin-10 (IL-10) were analyzed by enzyme-linked immunosorbent assay. Test group showed significantly better outcome compared to the control group in all clinical parameters (PI, GI, PD and CAL). The total amount of IL-10 increased after treatment in both test and control groups. From baseline to three months, the SRP with Diode laser group demonstrated a substantial rise in IL-10 levels, demonstrating how lasers can improve nonsurgical periodontal treatment results. Thus, diode laser when used in adjunct to SRP, the levels of IL-10 which is an anti-inflammatory cytokine, significantly increased which indicated reduced inflammation of the tissues.