Beech forests occupy considerable areas in the Bulgarian mountains. They are represented by communities of Fagus sylvatica (incl. F. moesiaca) and F. orientalis, and also by mixed stands of beech with Abies alba, Carpinus betulus, Quercus cerris, Q. dalechampii and Q. polycarpa. 494 relevés sampled across the country were analysed by numerical methods. They were classified into 12 groups of Fagus sylvatica forests and 3 groups of Fagus orientalis forests. The analysis of Bulgarian Fagus sylvatica communities did not show a distinct pattern of geographic differentiation and did not support the concept of the alliance Fagion moesiacum, as accepted by many earlier authors. The differentiation patterns in the Bulgarian Fagus sylvatica forests mainly follow the gradients in soils and climate, and are similar to those in the Central European beech forests. Therefore we accept a syntaxonomical scheme, which emphasizes variation due to edaphic and local topoclimatic factors rather than due to large-scale geographical differentiation. This scheme is very close to that proposed by Willner (2002) for the southern Central European beech forests, and includes the alliances Luzulo-Fagion (acidophilous beech forests), Asperulo-Fagion (nutrient-rich beech forests), and Cephalanthero-Fagion (thermophilous beech forests). The communities of Fagus orientalis are markedly different from the communities of Fagus sylvatica, have a distinct floristic composition, and belong to the Euxinian alliance Fagion orientalis.

Antioxidant capacity of the methanolic extract of Alchemilla mollis was measured by its ability to scavenge the DPPH radical. The EtOAc fraction obtained after partition of the total extract was found to be the most active radical scavenger (IC 50 9.8 ± 1.8 μg/mL) and was subjected to fractionation by Sephadex LH-20 CC. Further purification by RP-18 CC led to the isolation of eight flavonoid glycosides: cisand trans-tiliroside (1 and 2), rhodiolgin (3), hyperoside (4), isoquercitrin (5), miquelianin (6), sinocrassoside D 2 (7), and gossypetin-3-O--D-galactopyranosyl-7-O-α-L-rhamnopyranoside (8). It was found that 8 is a new compound and its antioxidant activity is also reported. Identification of the isolated compounds was carried out by spectroscopic and spectrometric analysis (1D and 2D NMR, UV and MS).

No abstract

No abstract