To further define the structure of the pericentromeric region of human chromosome 7, we have identified and characterized a YAC clone (YAC 311.H5) containing the D7S1480 locus, which maps to the short arm near the centromere of this chromosome, by linkage in CEPH families and radiation hybrid analysis. This YAC contains two new blocks of alphoid DNA (named Z5 and Z6). Both Z5 and Z6 show monomeric structures and a lack of higher-order repeats, and, therefore, belong to suprachromosomal family type 4 (M1). The orientation of the two blocks and the physical distances over the region were defined by pulsed-field gel electrophoresis (PFGE) and fluorescence in situ hybridization on chromatin fibers (FiberFISH). A YAC contig spanning the centromeric region has been developed by STS content.

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The objective of this paper is to describe on-going research on the seismic hazard analysis and vulnerability assessment for the Montreal Urban Community. The first part describes the overall work being performed in developing an inventory of critical lifelines and the estimation of their seismic vulnerability. The second part describes a procedure and the results for a seismic microzonation of site effects that combine both a field approach based on ground-ambient noise analysis and numerical modeling based on one-dimensional SHAKE computations.