Development of a single crystal sample holder for interfacing ultrahigh vacuum and electrochemical experimentation Review of Scientific Instruments 92, 074104 (2021);

Omnidirectional microscopy (OM) is an emerging technology capable of enhancing the threedimensional (3D) microscopy widely applied in life sciences. In OM, precise position and orientation control of the sample are required. However, current OM technology relies on destructive, mechanical methods to hold the samples, such as embedding samples in gel or attaching them to a needle to permit orientation control. A non-contacting alternative is to levitate the sample. Up until now levitation methods have lacked orientation control. We enable omnidirectional access to the sample by introducing a method to control acoustic levitation that provides precise orientation control. Such control around three axes of rotation permits rapid imaging of the sample from any direction with a fixed camera and subsequent 3D shape reconstruction. The control of non-spherical particles is achieved using an asymmetric acoustic field created with a phase-controlled transducer array. Our technology allows robust 3D imaging of delicate samples and their study in a time-lapse manner. We foresee that the described method is not limited to microscopy and optical imaging, but is also compatible with automated sample handling, light-sheet microscopy, wall-less chemistry, and non-contacting tomography.

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