A redox active polymer based on phenothiazine (thionine) doped poly (3,4-ethylenedioxythiophene) PEDOT film was examined on a range of transducers (glassy carbon, Pt and screen printed electrodes). This was followed by investigations into the use of super activated carbon (SAC) and platinized super activated carbon (SAC-Pt) nanostructured electrode modifiers for enhanced polythionine/PEDOT film deposition. The Polythionine/PEDOT film was found to undergo a two-electron, two-proton (pH 1-4) or a two-electron, one-proton process (pH 4-8). Electrochemical investigations included scan rate studies confirming the surface confined behavior, with the most stable films (15% decrease in electroactivity) being evident at SPE modified with SAC-Ptsurface coverage (Γ) 1.16 × 10 −10 mol cm −2 . Surface morphology of the formed film was investigated via SEM/EDX and film hydrophobicity examined via contact angle measurements.
A robust electrochemical assay for sorbitol dehydrogenase (SORDH) activity in milk was developed using voltammetry and chronocoulometry at bare and polymer modified transducers.
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