A cross sectional study on infectious bursal disease was conducted in apparently healthy backyard chicken at Waliso district of Southwestern Shoa, central oromia, Ethiopia from from November, 2018 to October, 2019. A total of 282 chickens were randomly selected to estimate seroprevalence of IBD infection and to identify the likely potential risk factors for the disease. Serum samples collected and serological test conducted in laboratory at National Animal Health Diagnosis and Investigation Center Sebeta, Ethopia. Out of 282 serum samples tested 224 were positive for indirect ELISA technique and the overall seroprevalence of IBDV in the study area was found to be 79.43% at individual level. Educational level of owners, kebeles and flock size significantly affect seroprevalence of IBD in the study area. The effect of difference in managements like source of replacement, frequency of house cleaning, use of disinfectant and isolation practice has a significant effect on IBDV sero-prevalence. A lower seroprevalence of IBDV was reported in good hygienic level of house (26.7%) than poor level of chicken house hygiene (96.4%) with statistically significant difference (P < 0.05). The seroprevalence of IBDV in the present study associated with chicken management, flock size, owner education level and other animal related risk factors for occurrence of the disease. Therefore, awareness on chicken health management, and importance of immunization would help to minimize the prevalence of the disease and play crucial role in the control of the disease. Furthermore, characterizing virus strains circulating in the area in future study is recommended.

Across-sectional study was conducted from November, 2014 to April, 2015 in selected dairy farms in Bishoftu Town to isolate and identify Staphylococcus aureus from milk samples of mastitis positive cows. The samples were taken from CMT positive cows and, isolation and identification of an organism was based on morphological, cultural and biochemical characteristics. A total of 46 lactating cows and 128 quarters were tested for mastitis using CMT, of which 13 (28.26%) sub-clinical mastitis at cow level and 21(16.40%) was at quarter level. Those CMT positive milk samples were collected and cultured parallel on both Blood agar and Nutrient agar. Out of the total 21 sub-clinical mastitis 18 samples were well grown on both Medias and sub cultured on nutrient agar for farther identification using Primary and secondary biochemical tests. Accordingly, 16 milk samples were shown typical yellow pigmentation on mannitol salt agar which is selective media for genus Staphylococcus. Finally, 9(42.85%) Staphylococcus aureus were identified on purple agar with the result of deep yellow pigmentation. Hence, implementing hygiene conditions, creation of awareness on control and prevention of subclinical mastitis in dairy farms and conducting drug sensitivity test for Staphylococcus aureus is recommended.

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