<p><em>Abstrak</em> <strong>- </strong><strong><em>Blood Disease Bacterium </em></strong><strong>(BDB) adalah penyakit pada banyak tanaman<em> </em>bernilai ekonomis seperti tanaman pisang yang disebabkan oleh infeksi soil-borne bakteri patogen <em>Ralstonia solanacearum</em>. Kemampuan <em>R.solanacearum</em> untuk bertahan pada kondisi minimum pada tanah dan luasnya spektrum inang pertumbuhannya termasuk vektor perantara, menyebabkan belum ditemukannya strategi efektif untuk menanggulangi penyebaran penyakit BDB. Pengetahuan tentang respons tanaman pisang terhadap serangan <em>Ralstonia solanacearum</em>, merupakan langkah awal untuk memahami stategi tanaman dalam membentuk sistem pertahanan. Dalam penelitian ini, respons ekspresi molekuler gen-gen terkait pertahanan pada tanaman pisang kepok (<em>Musa paradisiaca</em>) dianalisis menggunakan teknik semikuantitatif real time PCR. Data pada penelitian ini menunjukkan <em>WRKY15</em> memiliki penurunan ekspresi pada hari ke-3 dan ke-7 pasca infeksi. <em>WRKY18</em> memiliki ekspresi paling rendah dibandingkan dengan <em>WRKY15 </em>dan<em> WRKY23 </em>serta tidak terdeteksi lagi ekspresinya pada hari ke-5 pasca<em> </em>infeksi karena <em>WRKY18</em> bersifat represi pada pengaturan sistem pertahanan tanaman basal. Level ekspresi <em>WRKY23</em> memiliki pola yang konsisten pada hari ke-1 sampai hari ke-7 pasca infeksi karena <em>WRKY23</em> merupakan salah satu early induced gene. Penelitian ini juga didukung dengan data morfologi yang menunjukkan gejala penyakit layu bakteri berupa penguningan daun pada hari ke-5 pasca infeksi dan pada hari-4 pasca infeksi telah terlihat koloni R.solanacearum pada pseudostem.</strong></p><p> </p><p><strong><em>Kata Kunci </em></strong><em>-<strong> </strong></em><em>Transkriptomik, WRKY, Ralstonia solanacearum, Penyakit Layu<strong> </strong>Bakteri</em></p><p> </p><p><em>Abstract - <strong>Blood Disease Bacterium</strong></em><strong> (BDB) is a disease in many economically valuable plants such as banana plants caused by soil-borne infection of pathogenic bacteria <em>Ralstonia solanacearum</em>. The ability of <em>R. solanacearum</em> to withstand minimum conditions on the ground and the extent of its host growth spectrum including intermediate vectors, has resulted in the lack of effective strategies to combat the spread of BDB. Knowledge about banana plant response to <em>Ranstonia solanacearum</em> attack, is the first step to understand crop strategy in forming defense system. In this study, the response of molecular expression of defense-related genes in banana kepok plant (<em>Musa paradisiaca</em>) was analyzed using semi-quantitative real time PCR technique. Data in this study showed WRKY15 had decreased expression on. WRKY18 has the lowest expression compared to WRKY15 and WRKY23 and no longer detected its expression on the 5th post-infection day because WRKY18 is repressive on the regulation of basal plant defense system. The expression level of WRKY23 has consistent pattern on the 1st day until the 7th post-infection day because WRKY23 is one of the early induced gene. This study was also supported by morphological data showing symptom of bacterial wilt disease in the form of yellowing of leaves on the 5th day post infection and on day 4 post infection has seen colonies R. solanacearum on pseudostem.</strong></p><p><strong> </strong></p><strong><em>Keywords</em></strong><em> - Transcriptomic, WRKY, Ralstonia solanacearum, Blood Disease Bacterium</em>
Tujuan penciptaan yaitu untuk memvisualisasikan nilai sosial pendakian gunung sebagai sumber ide penciptaan seni lukis. Proses penciptaan berpedoman kepada metode penciptaan seni berbasis praktik (Practice Based Research). Lukisan ini disajikan melalui gaya surealis. Lukisan yang dihasilkan berjumlah 6 buah dengan berbagai ukuran yaitu kebersamaan dengan ukuran (100 x 120 cm), dan karya selanjutnya adalah terbongkar dengan ukuran (80 x 100 cm), sumber kehidupan dengan ukuran (80 x 100 cm ),siang malam dengan ukuran (80 x 100 cm) kesetaraan dengan ukuran (80 x 100 cm), semut kecil dengan ukuran (80 x 110 cm ). The purposes of creation is visualized the social value of mountain climbing as a source of painting creation ideas. The purposes of creation is guided by methods of practice-based research. This painting is presented in a surreal style. The paintings produced amounted to six pieces of various sizes, namely togetherness with sizes (100 x 120 cm), and the next work is uncovered with size (80 x 100 cm), source of life with size (80 x 100 cm), day and night with sizes ( 80 x 100 cm) equality in sizes (80 x 100 cm), small ants in sizes (80 x 110 cm).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.