The current study was conducted to determine the quality of semen a native chicken Kalimantan breed. A total of four cocks were used in this study. Semen was collected twice per week via the dorsal-abdominal massage method. Semen qualities were very important to the success of fertility in the chicken. The semen was then assessed macroscopic and microscopic to determine viability, motility, integrity of the membrane plasma and to look for abnormalities. There were no significant differences (P>0.05) in sperm viability, abnormality or integrity of the membrane plasma between treatments and the control before freezing. However, sperm motility before freezing was significantly different (P<0.05) between the added 4% of honey (P4 treatment) semen and the controls. Semen qualities post-thawing such as viability, motility, abnormalities and integrity of the membrane plasma were not significantly different (P>0.05) between the controls and all treatments. The motility of spermatozoa before freezing for the P4 treatment was significantly lower than that control and all treatments, and the addition of honey with 2, 4, 6, 8% has no effect on semen qualities in post-thawed chicken spermatozoa.