This work reports the analysis of cleaning solutions used in cleaning in place (CiP) and the changes that occur during storage at room temperature. Tests were performed on different solutions used to clean brewhouse vessels and a mash filter in a local brewery. The solutions were collected from CiP tanks after regeneration and were stored for three months in darkness at room temperature. Over time, the physical, chemical and microbiological parameters of the cleaning solutions were analysed. At the end of the study, the solutions were evaluated in terms of surface wetting and cleaning capability. The results showed that storage of the solutions at room temperature contributed to the agglomeration of particulates into larger conglomerates that may contribute to clarification of cleaning solutions by sedimentation. However, other parameters of the stored CiP solutions did not change. © 2019 The Institute of Brewing & Distilling