Background. Virgin coconut oil is a natural product from coconut that has many benefits such as antibacterial, anti-inflammatory, and antioxidant. Inflammatory disease of the periodontal tissues has a high prevalence worldwide. The main etiology of periodontitis plaque of biofilm contains colonies of pathogenic microorganisms. The occurrence of inflammation in the periodontal tissue stimulates the release of inflammatory mediators, such as TNF-α and TGF-β. Treatment for periodontitis can be performed starting from initial therapy and usually accompanied by additional therapy such as local drug delivery. VCO can be used as an alternative to antibiotics. Objective. To determine the effectiveness of VCO gel on periodontal tissue regeneration materials through the expression of TNF-α and TGF-β1. Methods. This is an experimental laboratory with a posttest-only control group design. VCO was made from grated fresh coconut and then mixed with NaCMC to obtain gel viscosity. The subjects of this study were 30 male periodontitis-induced Wistar rats by injecting Porphyromonas gingivalis into the gingival sulcus. Wistar rats were then divided into 3 groups. On the 7th and 14th days, the rats were sacrificed and the jaw was sampled to determine the amount of TNF-α and TGF-β1 expression in the regenerative process of periodontal tissue. Result. The amount of TNF-α and TGF-β1 increased significantly in the treatment group, but not as much as the increase in the positive control and negative control groups. Conclusion. VCO gel can affect the expression of TNF-α and TGF-β1 in the regeneration process of periodontal tissue in periodontitis-induced rats.

Platelet-rich fibrin (PRF) is the generation of platelet concentrates containing growth factors, which play a role in theprocess of healing and tissue regeneration. This study aimed to compare the effects of using of PRF with subepithelialconnective tissue graft (SCTG)as a treatment to gingival recession Miller Class I-II. Nine patients with bilateralgingival recession, as the subjects have two recessions. One side was treated with coronally advanced flap(CAF)+SCTG, while the other side was treated with CAF+PRF. The length of the recession is measured andcalculated as the percentage of root closure before and after surgery (10 and 30 days). The results showed that therewere significant differences in the average length of recessions between PRF and SCTG before and after the action onday 10 and day 30 (p<0.05). Closing percentage of gingival recession 10 days after SCTG treatment was 7 (77.8%)had experienced completely closing; while the PRF, only 6 (66.7%); p>0.05. Whereas on day 30, after SCTGtreatment, all subjects had undergone a recession closing perfectly, while the PRF group only 66,7% recession thathave closing completely (p=0.05). It was concluded that the CAF+SCTG provide maximum results compared withCAF+PRF in treating gingival recession Miller Class I–II.

Objective: Describes and compare the procedure of superior labial frenum frenectomies with conventional technique and incision below the clamp technique.Methods: Two female patient came to Departement of periodonsia, Unhas Dental Hospital to have frenectomies. The first patient was 28 year old with labialis superior frenulum reached attached gingiva, gingival recession 1-2 mm with calculus deposits, and referred to do frenectomy with Incision below the Clamp. While the second patient was 15 year old with labialis superior frenulum extend up to palatine papilla, central diastema and referred to do frenectomy with conventional technique.Results: The conventional techniques is done by engaged the frenum by a haemostat that inserted into the depth of the vestibule, and incision were placed on the upper and the under surface of haemostat, then followed by suturing the wound and periodontal pack. Insision below the clamp technique is done by placing a hemostat in position adjacent and parallel to the lip mucosa, and incision carried out below the clamp, then followed by suturing at the mucolabial fold and periodontal pack.Conclusions: Patients were very satisfied with the results that achieved. Technique Incision below the Clamp is an alternative treatment with good aesthetic and less bleeding during frenectomies by using a scalpel.

Objective : Periodontal disease is one of oral and dental diseases which most commonly found in humans caused by several factors, one of them due to the accumulation of bacterial plaque. Aggregatibacter actinomycetemcomitans is a bacterial pathogen that frequently causes periodontal disease.Material and Methods : To inhibit the growth of these bacteria can be done by using natural ingredients that contain anti-bacterial agent. One of the natural ingredients that contain antibacterial substances is Siwak. To determine the effect of siwak extract against bacteria Aggregatibacter actinobaciluscomnitans as one of the pathogenic bacteria causing periodontal diseases. The study was laboratory experimental with posttest only control group design.Results : This study used sample of Aggregatibacter actinomycetemcomitans colonies in MHA medium. Twenty four samples were divided into six treatment groups, they were 5 groups given the extract siwak with concentration of 3.125%, 6:25%, 12.5%, 25%, 50% and 1 group treated with control DMSO 5%. Inhibition zone was measured after 48 hours incubation at 37°C and measured using caliper. Data analysis was performed using Kruskal Wallis test. The mean diameter of inhibition zone at concentrations of 3.125%, 6:25%, 12.5%, 25%, and 50% were 6.4 mm, 7.0 mm, 7.2 mm, 7.9 mm and 8.6 mm.Conclusion : Siwak extract can inhibit the growth of Aggregatibacter actinomycetemcomitans as one of the pathogenic bacteria causing periodontal diseases with the largest concentration is a concentration of 50% and the smallest concentration that is 3.125%.

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