Purpose: The purpose of the present study was to isolate Pseudomonas sp. 2 from rhizosphere and screen for best amylase producer and to check for its suitability for industrial applications. Methods: Four strains of Pseudomonas were isolated from rhizosphere soil, by serial dilution method, of which Pseudomonas sp. 2 gave best results to α-amylase production. This strain was used to determine the enzyme production by SSF on wheat bran. Results and conclusion: The optimal enzyme production was obtained at 37 ºC and pH 7.5. Vitamins B 12 (50 mg) followed by Pyridoxine (50 mg) were found to be enhancing the enzyme production. Amino acids cysteine (30 mg), tyrosine (40 mg) and alanine (30 mg) were stimulating the enzyme production. The enzyme was partially purified by ammonium sulphate precipitation, and was further purified by ion exchange DEAE-cellulose column Chromatography. The purity of the enzyme was 45.2 fold greater than the crude enzyme. The molecular weight of the enzyme was found to be ~62 kDa by SDS-PAGE. This is the first report of any Pseudomonas sp. from rhizosphere being used for amylase production. The purified enzyme was not thermostable but it was acid tolerant making it suitable for industrial application. Key words: Pseudomonas sp. 2, α-amylase, SSF, Vitamins, Amino acids, Partial purification. Conflict of interest:The authors declare no conflicts of interest of any kind. ÖZETAmaç: Çalışamanın amacı Rizofer'den Pseudomonas sp. 2 bakterisini izole etmek, bundan en iyi amilaz üretimini bulmak ve bunun endüstriyel aplikasyonlara uygunluğunu kontrol etmektir. Metot: Seri dilüsyon metodu ile 4 tane Pseudomonas suju izole eidilmiştir. Bunların arasından Pseudomonas sp. 2 en iyi α-amilaz üretimini vermiştir. Bu suj, buğday kepeğinde SSF ile enzim üretiminde kullanılmıştır. Sonuçlar ve Tartışma: Optimum enzim üretimi 37 ºC and pH 7.5'da elde edilmiştir. Vitamin B 12 (50 mg) ve takiben piridoksin (50 mg) muamelelerinin enzim üretimini arttırdığı bulunmuştur. Sistein (30 mg), ıirozin (40 mg) ve alanin (30 mg) amino asitlerinin de enzim üretimini arttırdığı gözlenmiştir. Enzim, amonyum sülfat presipitasyonu ve DEAE-selüloz kolon kromatografisi ile kısmen saflaştırılmıştır. Saflaştırma verimi, ham enzimden 45.2 kez fazladır. SDS-PAGE kullanılarak enzimin molekül ağırlığı ~62 kDa bulunmuştur. Bu çalışma, Rizosfer'den elde edilen Pseudomonas sp.'dan ilk defa amilaz elde edilmesi ve kullanılmasını sunmaktadır. Kısmen saflaştırılmış enzim termostabil değildir, fakat aside dayanıklıdır ki bu enzimi endüstriyel uygulamalar için uygun hale getirmektedir. Anahtar Kelimeler: Pseudomonas sp. 2, α-amilaz, SSF, Vitaminler, Aminoasitler, Kısmi saflaştırma. Çıkar çatışması: Yazarlar hiçbir çıkar çatışması bulunmadığını beyan ederler.

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The present communication deals with the ethno-botanical exploration, identification of some plants of formerly Asclepiadaceae that are treated as subfamily Asclepiadoideae, the milkweed subfamily of family Apocynaceae (order Gentianales) as per APG III system of classification. The plants of this subfamily are being used frequently by the rural people of Bankura. During the present investigation, a total of 14 species of subfamily Asclepiadoideae have been reportedthey were used by the local health healers for the treatment of different diseases. Among them Calotropisgigantea, Hemidesmusindicus, Pergulariadaemia, Telosmapallida, Tylophoraindica, are leading species that are frequently used for a variety of health problems. As reported by informants, roots are the most important part used for herbal preparation followed by whole plant, leaf and fruit. The rural people of Bankura have good knowledge about ethno-medicinal plants and this legacy of traditional culture must be conserved. So present paper depicts various plant resources of ethno-medicinewith special reference to subfamily Asclepiadoideae.

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