Background: Ticks and tick-borne diseases (TBDs) are considered to be the most serious health problems for animals and humans. This issue prompts veterinarians and researchers to adopt better preventive and control strategies. Therefore, this study aims to develop hybridoma vaccine against Hylomma dromedarii infesting Camelus dromedaries. Methods: Myeloma cell culture was administered to the immunized mouse in order to produce hybridomas cells. These cells were then emulsified with Montanide ISA-51 adjuvant. Two doses of the prepared anti-tick vaccine were administered intradermally to camels with an interval of five weeks. The humoral response of experimental animals was measured by indirect enzyme-linked immunosorbent assay (ELISA). Result: There was a significant (p less than 0.05) decrease in immune responses in tick counts and molting capacity. Overall, 85% efficacy of the developed vaccine was recorded. It is concluded that hybridoma based vaccine is effective in controlling H. dromedarii infestation against camels.

Malaria is one of the major health problem caused by a vector borne disease of the genus, plasmodium. This parasite is transmitted through bite of female mosquito, Anopheles called malarial vector. The present work was conducted during 1 st August 2017 to 31 st July 2018 to examine the frequency rate of malarial infection in school going children of Quetta (city) A total no. of 2213 blood slides were prepared (thin & thick) from 33 different schools of Quetta (city) visited twice in each month out of 2213/433 are positive for the malaria infection. The overall positivity rate was (19.5%). However P. vivax was found to be the most common specie with the highest frequency rate (84.52%) as compared with that of P. falciparum (6.01%) and mixed infection was also seen in the present study (12.29%). The variations were found in different age groups. The higher frequency rate of P.vivax (90.8%) in the age group of 11-16 years and lowest was found (81.4%) in the age group of 3-5 years.