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Agroforestry has been suggested as a sustainable strategy to promote biodiversity conservation in agricultural land, and farmers' knowledge can be crucial to design more diverse agroforestry systems. Therefore, the main objective of this study was to assess the conservation value of agroforestry systems in relation to forest areas in different stages of natural succession as well as farmers' knowledge. The conservation value of agroforestry systems (coffee and pastures) was assessed through tree diversity indices and compared with values found in forest areas using rarefaction curve. In parallel, we assessed the tree functions according to farmers' knowledge using an ethnobotanical survey. Species richness in