Breast cancer is considered as a leading cause of cancer related death worldwide. The development of chemotherapeutic resistance and the production of undesirable side effects steer the search for new potential candidates with therapeutic implications. Lantana camara has been reported to cure a number of ailments, with few studies showing its cytotoxic effect on breast cancer cell lines. However, the impact of Lantana camara on triple negative breast cancer cells is largely obscure to date. The present study investigated the effect of ethanolic extract of Lantana camara leaves on the triple negative breast cancer cell line, MDA-MB-231. We found that Lantana camara leaf extract induced cytomorphological changes and exhibited a growth inhibitory effect on MDA-MB-231 cells in a dose-dependent manner. The extract was observed to induce G0/G1 cell cycle arrest. Nuclear staining of the cells exposed to Lantana camara leaf extract suggested the presence of condensed nuclei and the result of flow cytometry analysis confirmed cell death by apoptosis. Lantana camara leaf extract also reduced the migration of MDA-MB-231 cells as evidenced by the wound healing assay results. In summary, this study demonstrates the efficacy of the extract to produce growth-inhibitory and anti-migratory effects on MDA-MB-231 cells. Our results thus suggest that Lantana camara leaf extract can be considered a potent source of chemotherapeutic agents for triple-negative breast cancer treatment.