The eyes function through the cooperation of different ocular components, and complications with any components would lead to ocular diseases that deteriorate vision. Hence early and precise detection and monitoring of ocular diseases, along with the improved understanding of pathological mechanisms, becomes essential for successful treatment. Photoacoustic Imaging (PAI) is a non-invasive and non-destructive imaging modality based on the photoacoustic effect, which gives high spatial resolution, sensitivity, contrast and penetration depth. Since PAI can provide anatomic and functional ocular characterizations, it can be a potential tool for medical screening/diagnosis of ocular diseases, staging, treatment, and continuous post-treatment monitoring. Here, we perform deep-tissue imaging of the Goldfish (Carassius auratus) eye using a home-built Photoacoustic microscope to identify various ocular components like iris, crystalline lens, retina, optic nerve and blood vessels. The study can be extended to observe changes in these structures under different ophthalmic disease conditions.
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