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Mediterranean countries produce up to 97% of the total global olive production. Besides being a serious environmental burden, olive mill waste represents a potential resource of useful materials for recovery and valorization. In this work, the adsorption characteristics and potential of bioadsorbent material manufactured by alkali treatment of olive cake was evaluated. The resultant bioadsorbent was able to purify biodiesel to meets both ASTM D6751 and EN 14214 standards. Further, the sorption equilibrium was investigated at 25 °C. Langmuir, Freundlich, and Temkin isotherms fit to the experimental data were evaluated by applying the non-linear Chi-square test. Freundlich and Temkin isotherms were shown to adequately described the adsorption isotherms of the produced bioadsorbent.