ARE cell clones can provide a useful in vitro model to study the mechanism of SP6-mediated amelogenesis imperfecta.

No abstract

<p>End-stage renal disease (ESRD) is one of the public health problems in Indonesia. This study aimed to determine the clinical condition of premorbid and the incidence of ESRD. The study design is a case-control. The number of volunteers involved in this study was 100 patients. The cases were ESRD patients who were determined by the criteria of Pernefri and the controls were patients from the same hospital who were not ESRD by the requirements of ESRD Pernefri. Inclusion criteria of the study sample were ESRD sufferers need routine hemodialysis and doctor's diagnosis supported by laboratory data, 15-75 years old and willing to fill out inform consent. Exclusion criteria for the study sample were patients with congenital kidney disease, Have a history of kidney transplants, and mental disorder. We collected demographic data, and patient’s clinical and drug history from medical records. Chi-square analysis with 2x2 tables and the Fisher's exact test were used to determine the relationship of clinical conditions and history of disease with the incidence of ESRD. The results showed that anemia, hyperglycemia, and hypertriglyceridemia were related to the prevalence of ESRD. History of hypertension, diabetes mellitus, dyslipidemia, and family history of ESRD are associated with an increased incidence of ESRD.</p>

Tooth development relies on the interaction between the oral ectoderm and underlying mesenchyme, and is regulated by a complex genetic cascade. This transcriptional cascade is regulated by the spatiotemporal activation and deactivation of transcription factors. The specificity proteins 6 (Sp6) and chicken ovalbumin upstream promoter transcription factor-interacting protein 2 (Ctip2) were identified in loss-of-function studies as key transcription factors required for tooth development. Ctip2 binds to the Sp6 promoter in vivo; however, its role in Sp6 expression remains unclear. In this study, we investigated Sp6 transcriptional regulation by Ctip2. Immunohistochemical analysis revealed that Sp6 and Ctip2 colocalize in the rat incisor during tooth development. We examined whether Ctip2 regulates Sp6 promoter activity in dental epithelial cells. Cotransfection experiments using serial Sp6 promoter-luciferase constructs and Ctip2 expression plasmids showed that Ctip2 significantly suppressed the Sp6 second promoter activity, although the Sp6 first promoter activity was unaffected. Ctip2 was able to bind to the proximal region of the Sp6 first promoter, as previously demonstrated, and also to the novel distal region of the first, and second promoter regions. Our findings indicate that Ctip2 regulates Sp6 gene expression through direct binding to the Sp6 second promoter region. J. Med. Invest. 61: 126-136, February, 2014.

Kesehatan gigi dan mulut merupakan bagian dari kesehatan tubuh yang tidak dapat dipisahkan satu dengan yang lainnya sebab kesehatan gigi dan mulut akan mempengaruhi kesehatan tubuh keseluruhan. Menurut data Riskesdas 2018 sebanyak 57,6% penduduk Indonesia bermasalah gigi dan mulut selama 12 bulan terakhir, tetapi hanya 10,2% yang mendapat perawatan oleh tenaga medis gigi. Hal tersebut dapat terjadi karena masih rendah-nya motivasi masyarakat dalam menjaga kesehatan gigi dengan perawatan dan pemeriksaan rutin ke tenaga kesehatan. Masyarakat cenderung akan datang, jika sudah dalam kondisi sakit. Rendahnya motivasi tersebut dipengaruhi oleh kurangnya pengetahuan masyarakat terkait dengan masalah kesehatan gigi dan mulut. Berdasarkan telah terbentuknya kader promosi kesehatan gigi dan mulut di wilayah Kecamatan Srandakan yang bekerja sama dengan Posbindu Bisma Remaja Masjid Islahul Umam Dusun Talkondo, pada tahun ini pada pelaksanaan tahun kedua diwilayah tersebut melakukan kegiatan dengan program implementasi pengukuran kesehatan gigi anggota binaan Posbindu Bisma. Selama kegiatan berlangsung, dilakukan pengambilan nilai pra perlakuan dan pasca perlakuan untuk mengetahui tingkat kesehataan gigi anggota binaan. setelah dilakukan analisa statistic, hasil nilai Asymp. Sig. (2- tailed) sebesar 0.001. Dari nilai Asymp. Sig yang diperoleh, dapat diartikan bahwa terdapat perbedaan nilai kesehatan gigi antara pra perlakuan dengan pasca perlakuan (sig. <0.05). Hasil tersebut dapat menunjukkan informasi, bahwasannya perlakuan yang diberikan kepada anggota binaan memiliki pengaruh.