Koi herpesvirus specifically infects and causes mass mortality on koi and carp, resulting in severe economic losses. In this study, we presented the efficacy of KHV DNA vaccine administration by immersion method on Cyprinus carpio. Two different immersion densities of fish were applied, namely 800 fish L À1 and 1200 fish L À1 . Thirty-day-old common carp juveniles were immersed for 30 min in the water containing 1.3 9 10 8 CFU mL À1 of heat-killed Escherichia coli carrying DNA vaccine encoding glycoprotein-25, and without vaccination treatment as controls. The challenge test was performed at 30 days post vaccination by injecting 0.1 mL KHV filtrate (10 À3 of dilution rate). The result showed that higher relative per cent survival of KHV-challenged fish was obtained in 800 fish L À1 (P < 0.05). Furthermore, significant specific antibody anti-KHV response (P < 0.05) was detected on 28 and 36 days post vaccination in 800 and 1200 fish L À1 , respectively, compared to the controls there was no specific antibody detected. In conclusion, the KHV DNA vaccine could provide good protection in common carp against KHV infection, which has practical applications in aquaculture practices.

With the high demand for sea lobster as the industry has developed, Indonesia has an opportunity to establish the world's largest lobster aquaculture production. Therefore, a study related to the growing-out of lobster and also the development of engineering technology of lobster enlargement is needed. This study aims to determine the growth performance of Panulirus homarus reared in a bottom cage method. Lobsters with an average size of 116,25 g were reared for 150 days and fed with trash fish of 3%-4% of the biomass daily. The results showed that the use of the bottom cage method had positive effects on the survival rate, average body weight, specific growth rate dan food conversion ratio was 85%, 170 g, 0.25 g per day and11.16 respectively. The water quality is also was still within the suitable range for lobster rearing.

The vannamei shrimp farming business still has the opportunity to continue to develop given the public's high demand and high national shrimp production target that must be met. Vannamei shrimp is also a commodity that has high economic value. However, vannamei shrimp cultivation is known as cultivation which requires the cultivators to have large capital making it difficult for all groups to reach. The purpose of this community service activity is to provide information and insight to the public regarding shrimp farming with technology that can be applied on a small scale with relatively low capital and is expected to be accessible to all people who wish to cultivate vannamei shrimp using a household scale cultivation system (Backyard Shrimp Farming/BSF). This service activity is in the form of online dissemination using the zoom application and broadcast live via YouTube of the Politeknik Kelautan dan Perikanan Sidoarjo. The activity was carried out by delivering material by the speaker and followed by a discussion and question and answer between the participants and the speaker. At the end of the activity, participants were given a link to fill out an assessment questionnaire related to the dissemination activities carried out. Based on the results of the questionnaire, it can be concluded that the participants had a great interest in this household-scale vannamei shrimp farming opportunity and felt the benefits of this dissemination activity, and overall the dissemination had been carried out well in terms of clarity of material delivery, the interaction between participants and the speaker, the suitability of the material and the performance of the moderator/facilitator in presenting the event.

Tujuan dari penelitian ini adalah untuk menentukan tingkat subtitusi tepung ikan dengan tepung maggot sebagai sumber protein yang dapat menghasilkan pertumbuhan yang baik pada ikan nila (O. niloticus) serta mampu meningkatkan status kesehatan ikan nila. Pemeliharaan ikan dilakukan selama 28 hari. Rancangan percobaan yang digunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan dengan iso protein 33% dan iso energi 2.877,8 kkal/kg. Empat perlakuan substitusi tepung maggot terhadap ikan yang berbeda, meliputi perlakuan A (100%:0%), B (75%:25%), C (50%:50%), D (25%:75%). Hasil penelitian menunjukkan bahwa penggunaan substitusi tepung maggot dalam pakan tidak memberikan pengaruh terhadap kelulushidupan dan efisiensi pakan pada ikan nila, tetapi berpengaruh sangat nyata terhadap laju pertumbuhan spesifik 1,99±0,10; dan untuk jumlah konsumsi pakan 1125,51±1,88. Tidak ada perngaruh terhadap gambaran darah, baik kadar hemoglobin, sel darah merah dan sel darah putih pada ikan nila. Berdasarkan penelitian ini dapat disarankan bahwa penggunaan tepung maggot sebagai sumber protein dalam formulasi pakan ikan Nila (O. niloticus) dapat disubtitusikan sebesar 75% terhadap tepung ikan.