A field experiment was conducted during winter season of 2015-16 to study "Studies Effect of Planting Dates and Varieties on Growth and Quality on Gladiolus (Gladiolus hybridus Hort.) under sub-humid zone of Rajasthan" having 15 treatment combinations of five Varieties (V 1-African Star, V 2-Hunting Song, V 3-Legend, V 4-Pusa Srijana and V 5-Snow Princess) and three planting dates (D 1-10 th October, D 2-25 th October and D 3-9 th November). The treatment V 2 D 1 (Hunting Song + 10 th October planting) recorded the maximum plant height (116.10 cm), highest number of leaves per plant (8.77), main stem diameter (1.50 cm), number of florets per spike (15.8), spike length (91.80 cm), rachis length (57.90 cm), floret diameter (10.04 cm), vase life of spike (13 days), While the maximum leaf length (64.20 cm), leaf width (4.96 cm) in (V 5 D 3) and maximum spike girth (0.91cm) was found in (V 1 D 2). The growth and flowering quality was found superior in compare to other planting dates.