Diadenosine polyphosphates (diadenosine 5,5ٟ-P 1 ,P npolyphosphate (Ap n A)) are 5-5ٟ-phosphate-bridged dinucleosides that have been proposed to act as signaling molecules in a variety of biological systems. Isothermal titration calorimetry was used to measure the affinities of a variety of metal cations for ATP, diadenosine 5,5ٟ-P 1 ,P 3 -triphosphate (
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