Blood is the vital fluid which carries oxygen, nutrients, hormones, vitamins and antibodies to the tissues in the body and sends away the carbon dioxide and waste matters formed in the tissues from the body. Aim of the study: to assess knowledge and attitude among nursing students regarding blood donation. Research design: A descriptive research design was used for this study. Sample: A Stratified random sample, from first, second, third & fourth academic year students in Faculty of Nursing Minia University. Data collection Tool: three tools were used in the study: Tool I: Demographic characteristics of nursing students and their previous history, Tool II: Knowledge Assessment Tool, and Tool III: Blood donation Attitude Scale. Results The current study found that more than half of nursing students aged between 20-21 years with mean ± SD 20.05 ± 1.3 years, about three quarter of them was female, more than one quarter of them were in 3 rd year academic years and more than two third of them comes from rural area. About more than half of nursing students had unsatisfactory knowledge about blood donation and most of them had positive attitude about blood donation. Conclusion: About more than one third of nursing students had satisfactory knowledge about blood donation and most of them had positive attitude about blood donation. Also, there was statistically significant difference between nursing students' knowledge and their age, academic years and attitude toward blood donation. Recommendation: Increase knowledge of nursing students about blood donation through making a seminar and workshop about it.