The study include effect therapeutic dose of metformin drug in blood sugar of pregnant mice treated with folic acid. Mice weight ranged (25±2) g with age (12-16)weeks ,The concentrations of uesd were (0.18 & 0.53) mg \ 25 gm mice body weight which corresponded to (500&1500) mg\70 Kg of body weight in adult human , The therapeutic dose of folic acid used was (0.002) mg\mice body weight which corresponded to (5) mg \ body weight in human , was aimed to define the effects of drugs in sugar level during pregnancy with the presence of folic acid or its absence , The drug was orally administrated , at (6-18) th day of pregnancy while folic acid was administrated at (4-18) th day. This group was treated with metformin drug after 2 hours of folic acid admimstration at (6) th day , The administration was once in the morning of each , The results showed non significant decrease in the sugar level in groups treated with metformin while significant increase in groups treated with metformin drug and folic acid compared with control.

The study included the effect of metformin drug over dose in the liver pregnant non-Diabetic mice with weight ranged (25±2) gm , Orally administration of (1.8 , 2.85) mg of drug from (6-18) th day of pregnancy in the morning and once time daily, Drug caused death in half number of animals in 8.1)) mg concentrate and caused significant increase in liver weight in high concentration 2.85 mg and non significant in low concentration compared with control group (non treated) , In addition some histologically changes that included inflammatory cells around damaged blood vessels which increase with high dose caused primary biliary cirrhosis. Other changes include Degeneration , necrosis , Blood vessels damage ,Greenish, Congestion and hemorrhage and fibrosis in the liver of dead mice .