Sponges are often used as a reference for pollutant bioindicators, (pollution of heavy metals and poly aromatic hydrocarbons. Porosity of the sponge body is used as host microorganisms, especially bacteria and fungi. Phenotypic characterization and symbiotic genotypes of Niphates sp. molecular rRNA gene 2 of Nephates sp as symbionts isolates showed a positive reaction to methyl red (acid producer), VR, citrate (has a genetic carrier enzyme), lactose and catalase (acidic). This data shows that both isolates of Niphates sp can make carbon as a nutrient source of energy: Genotypic analysis of 2 isolates of Nephates sp in the phases of amplification and alignment of the sample gene homolog series on the Bank gene concluded that the 2 isolates were Bacillus cohnii strain DSM 6307 (BC) and Bacillus pumilus strain GLB197 (BP). (Fenantrena) shows growth cell cells based on parameters of increased optical density, gas bubbles formed, changes in media pH and fermentation odor arises.
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