Herein, we report for the first time, the synthesis of silver nanoparticles-nitrogen doped graphene oxide-chitosan nanocomposite (SGC) from the aqueous leaf extract of Curcuma caesia plant and we explored bacterial toxicology behavior of SGC nanocomposite via agar disc diffusion method. Curcuma caesia is a medicinal herb utilized as a reducing agent for the reduction of silver-to-silver ions. Graphene oxide is a significant two-dimensional carbon nanomaterial, possessing excellent physical, chemical, and electrical properties that make them unique for various applications. The Ultrasonication process at room temperature was utilized for the synthesis of SGC nanocomposite. The addition of biopolymer; chitosan, and silver nanoparticles (AgNPs) to the matrix of nitrogen-doped graphene oxide (NGO) was confirmed via various spectroscopic techniques such as FTIR, FT-Raman, XPS, and so on. Finally, the results of the disc diffusion method showed SGC nanocomposite exhibit concentration-dependent inhibition toward bacterial growth.