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This study, include the green synthesis of ZnO nanoparticles using ethanol extract of (Zingiber officinale) ginger was investigated in order to evaluate its antibacterial effect on fresh chicken meat. Determining the microbiological quality of meat involves performing various plate counts indicators include, including total bacterial count (TBC) and the total coliform counts TCC the UV-Visible Spectrophotometry and FTiR (Fourier Transform infrared spectroscopy) were used to characterize the GnZnO nanoparticles. A total of 180 samples of fresh chicken meat were subjected to (0.5, 1, and 1.5) mg/ml of GnZnONPs and bacteriologically examined was done. significant absorption of UV region at peak370nm verified the presence of ZnO NPs. The FT-IR spectra recorded the existence of biomolecules responsible for the reduction and capping of the green synthesized, Also observed result of a meat sample treated with GnZnONPs showed a significant (p < 0.05) reduction in (TBC) through 2, 5, and 7 days of storage at 4°C (1.5 mg). While the highest count recorded in control (untreated samples) reached 11.360.04 CFU.g-1 at the end of the experiment, the TCC also decreased significantly (p< 0.05) after 5,7 days of storage at 40 C when the meat samples were treated with GnZnONPs compared to the control sample. The findings show that treating poultry meat with GnZNoNPs extends the shelf-life of the meat, and maintains its quality throughout the storage period

Aqueous and ethanolic extracts of ginger )Zingier officinale) and (Cinnamomum zeylanicum) were used to investigate an antifungal activity against food spoilage fungal pathogens. Study of the inhibitory effect of these extract was done separately against Aspergillus niger and Penicillium notatum isolated from (tomato pasts, cheese) the samples were cultured on Sabouraud's dextrose agar (SDA) at pH 5.6 and 28°C. In this study, results had shown that the extracts of Both plant demonstrated antifungal activity. When compare extracts of the two plants, C. zeylanicum extracts showed higher inhibition activity than (Zingiber officinale) extracts significantly. Ethanolic extracts of these tow plants gives significantly inhabitation in the mycelia growth of fungi more than their aqueous extracts (16-22), (19-25)mm, P. noitatum and A. niger respectively while the inhibitory observed by the aqueous extract (5-6) mm against A. niger for (6-8) mm. Against P. notatum was the most affected by all extracts while A. niger was the least susceptible. There was a significant difference (P=0.05) between groups in the antifungal activities of tested fungi, and the effectiveness of ethanolic extracts was increased with increase in their concentrations. The ability of these extracts to inhibit the growth of the two fungi must take into consideration an indication of the antifungal potency of cinnamon and Zingiber officinale, that makes them the candidate for the production of antifungal agents.

Salmonella enteritidis is an important foodborne pathogen that affects the human health by conception  animal products and it was  considered as a worldwide public health hazard. This study was conduct to investigated the prevalence  of  S. enteritidis, in calves with diarrhea  in farm of AL- Diwanihyia city basis of molecular properties of this bacterium .  A total of 91 samples were collected from December 2013 to May 2014, in different farms in the al Diwanihyia  city . the pathogen    was isolated by  using microbiological and biochemical tests. the  DNA extraction was done  by genomic DNA kit according to the manufacturer’s instruction (USA) and PCR was performed via the specific primers of SefA- -F and SeA-R of the SefA gene. Amplified fragments of the 210 bp were observed in 12 of the total 91 stool samples  isolates. This study recommends that the identification of these pathogens by PCR technique can be replaced with traditional bacteriological tools. The PCR method is a rapid approach for recognizing and identifying the S. enteritidis infections in diarrheic calves.