Objective: To determine the antioxidant and anti-inflammatory activity of myco-synthesized silver nanoparticle (AgNP) from Pleurotus florida.Methods: Fresh mushrooms were collected, processed, and the AgNPs were synthesized using standard reducing agent 1 mM of silver nitrate. The characteristics of synthesized particles were confirmed using ultraviolet, Fourier transmission infrared, X-ray diffraction, and energy dispersive X-ray analysis. These nanoparticles were subjected for antioxidant activity through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and anti-inflammatory activity using bovine serum albumin denaturation assay, respectively, and the percentage of inhibition of AgNPs was calculated with standard error mean. Results:The AgNPs were recorded the absorbance band at 410 nm was the particle size of 20-50 nm with a rod in shape. In DPPH assay the antioxidant activity was recorded as 96.2% with 50% inhibitory concentration (IC50) value of 85 µg/ml. In anti-inflammatory activity, the activity was recorded as 92.10±0.005% and IC50 value was recorded as 100 µg/ml. Conclusion:The results indicated the significant antioxidant and anti-inflammatory activity of AgNPs from P. florida. Hence, further in vivo studies are focused to confirm the anticancer and anti-inflammatory activities.