Aim: To measure the prevalence of hepatitis C virus (HCV) among blood donors attending Samarra’a General Hospital. Patients and Methods: A questionnaire was developed in line with the study objectives. It was administered to blood donors visiting blood bank. After they were administered, informed consent and assurance with regards to confidentiality were provided. Results: A total of 50 blood donors were surveyed. The majority were males (66%) and most of them (40%) were between 21-35 years old. The majority were married (64%) and with intermediate education (46%). (44%) of the blood donors had transmitted their disease (hepatitis C) to their life partners and family members. (82%) of the blood donors whom we surveyed were having a positive history of receiving blood for certain conditions. Surgery was the common risk factor among the surveyed blood donors with (62%). Conclusions: Among blood donors, 66% of them were males and then the majority of blood donors 82% with the previous history of blood transfusion were infected with HCV. In addition, regarding risk factors for HCV; the common risk factor was the surgery which was 62%.