This study aimed to investigate the effect of natural multiple-enzyme supplementation on growth performance, gut microflora, carcass characteristics, and the meat quality of broilers reared in tropical climates. This study employed a completely randomized design (CRD). One thousand Ross 308 ® one-day-old chicks were divided into two treatments, each with 10 replicates and 50 chicks (25 females and 25 males) per experimental unit. Dietary treatment consisted of commercial broilers diets (corn-soybean based diet) supplemented with 0 or 100 g/ton of natural multi-enzymes feed supplementation (top-up feeding). Birds were provided ad libitum access to water and experimental diets. The results showed that natural multi-enzyme supplementation had no significant effect on the growth performances of broilers as compared to the control (P > 0.05). However, supplementation of natural multi-enzymes increased the viability rate of broilers during the starter period (P < 0.05). In addition, natural multi-enzyme supplementation exhibited positive effects on the productivity index, salable bird return, net profits return per bird, return on investment, and feed cost per gain compared to the control group (P < 0.05). Likewise, supplementation of natural multiple enzyme had no effect on the viscosity of the duodenal content and cecal microbial population, of broilers (P > 0.05). In addition, dietary treatments had no significant changes on carcass composition and meat quality (P > 0.05). Inclusion of 100 g/ton feed of natural multi-enzymes supplementation (top up feeding) of broiler chicken significantly affected the economic return of broiler production.