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Abstract. The ship dismantling industry is a cause of contamination of the environment by diesel. The objectives of this study were to isolate and screen diesel degrading bacteria from diesel contaminated areas. Diesel contaminated seawater and soil samples were collected from a ship dismantling facility at Tanjungjati, Madura, Indonesia. Isolation was conducted with an aseptic technique and growing the mixture culture was carried out based on the pour plate method. After 24 h of incubation, thirteen bacteria strains were isolated from diesel contaminated seawater and soil samples from the area of study. The isolated bacteria were identified based on morphological characterization. Mostly gram positive bacteria were found. The isolated bacteria were screened by using nutrient agar medium containing various diesel concentrations (0%, 5%, 10%, and 15% (v/v)). The result of the screening test showed that the bacteria coded EL and CT displayed the best resistance and highest growth in diesel polluted medium. It was shown that both of them potentially have a higher capability of utilizing diesel as carbon and energy source than the others.

Kebutuhan manusia terhadap air minum yang layak konsumsi semakin lama semakin berkembang sebanding dengan perkembangan penduduk yang kian lama kian meningkat. Untuk dapat memenuhi kebutuhan air minum sehari-hari, banyak industri yang membuka usaha air minum diantaranya adalah adanya depot air minum isi ulang (DAMIU). Pemilihan depot air minum isi ulang sebagai alternatif air minum menjadi risiko yang dapat membahayakan kesehatan jika kualitas depot air minum isi ulang masih diragukan. Penilitian ini menggunakan metode FMEA (Failure Mode and Effect Analysis) untuk mencari prioritas terjadinya kegagalan dalam proses pengolahan air minum isi ulang. Risiko-risiko yang didapat harus memiliki nilai agar terukur sehingga ditemukan prioritas penanganan dan dicari upaya untuk perbaikan. Penilaian tersebut dikenal dengan nilai RPN (Risk Priority Number) yaitu hasil pengalian dari tingkat keparahan (severity), frekuensi kejadian (occurrence) dan tingkat deteksi (detection). Berdasarkan hasil analisa dan identifikasi menggunakan metode FMEA diketahui bahwa kegagalan yang terjadi pada sistem UV dengan 3 prioritas tertinggi yaitu penggunaan UV dengan nilai RPN 100, waktu kontak UV dengan nilai RPN 100 dan penggantian filter awal dengan nilai RPN 64. Pada sistem UV dan ozon, 3 prioritas tertinggi yaitu waktu kontak sistem ozon, waktu kontak UV dan penggunaan UV dengan masing-masing nilai RPN yaitu 100. Sedangkan pada sistem UV, ozon dan RO (Reverse Osmosis) 3 prioritas tertinggi sama seperti dengan sistem UV dan ozon dengan nilai RPN yang berbeda yaitu lama pengoperasian dengan nilai 100, waktu kontak dan kesesuaian penggunaan UV dengan nilai 64.

The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe2+) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe2+) in further investigation.

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