Employee job performance is a critical topic of interest and concern for the organization. In order to evaluate the factors that may impact employee job performance in the context of COVID-19 pandemic, this study aims to explore the impact of absorptive capacity for e-Learning on the job performance of teaching faculty. Additionally, the study explored how organizational culture may act as a mediator. The COVID-19 pandemic calls for a transformation in the educational system from the physical to the virtual, which requires teaching faculty to increase their E-learning proficiency. It was necessary to assess each faculty member's effort in this situation. An analytical cross-sectional study design was used to collect data from the teaching faculty of higher education institutions in Karachi, Pakistan. The sample size was 377. Data was collected through reliable and valid self-administered tools. SPSS 23.0 and the PROCESS macro were used to conduct data analysis. Empirical findings from the study demonstrate the significant positive effect of knowledge recognition, assimilation, transformation, and exploitation on job performance. Furthermore, organizational culture significantly mediates the effect of knowledge recognition, assimilation, transformation, and exploitation on job performance. The findings have important implications for managers and policymakers besides employees in creating opportunities for their employees to develop high absorptive capacity and recommend managers to create an appreciative climate within their organizations, which may influence job performance.