Sub-Saharan agriculture has been identified as vulnerable to ongoing climate change. Adaptation of agriculture has been suggested as a way to maintain productivity. Better knowledge of intra-specific diversity of varieties is prerequisites for the successful management of such adaptation. Among crops, root and tubers play important roles in food security and economic growth for the most vulnerable populations in Africa. Here, we focus on the sweet potato. The Sweet potato (Ipomoea batatas) was domesticated in Central and South America and was later introduced into Africa and is now cultivated throughout tropical Africa. We evaluated its diversity in West Africa by sampling a region extending from the coastal area of Togo to the northern Sahelian region of Senegal that represents a range of climatic conditions. Using 12 microsatellite markers, we evaluated 132 varieties along this gradient. Phenotypic data from field trials conducted in three seasons was also obtained. Genetic diversity in West Africa was found to be 18% lower than in America. Genetic diversity in West Africa is structured into five groups, with some groups found in very specific climatic areas, e.g. under a tropical humid climate, or under a Sahelian climate. We also observed genetic groups that occur in a wider range of climates. The genetic groups were also associated with morphological differentiation, mainly the shape of the leaves and the color of the stem or root. This particular structure of diversity along a climatic gradient with association to phenotypic variability can be used for conservation strategies. If such structure is proved to be associated with specific climatic adaptation, it will also allow developing strategies to adapt agriculture to ongoing climate variation in West Africa.

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Le bananier représente l'une des cultures les plus importantes dans le monde. Au Togo, la culture de bananiers plantains se fait surtout dans la Région des Plateaux à l'Ouest dans de petites exploitations agricoles ; ce qui a entrainé une faible productivité. Cependant, les variétés de bananiers plantains cultivées ne sont pas bien connues. Un inventaire a été entrepris dans le but de dénombrer les variétés de bananiers plantains cultivés grâce à des paramètres agromorphologiques et aussi de montrer l'intérêt que suscite la valorisation de la culture de cette plante. Au terme de cette étude, cinq (5) variétés de bananiers plantains ont été recensées. La variété 'Apim' est la plus productive suivie de la variété 'Abladzo'. Contrairement aux autres, la variété 'Taévé' produit 2 régimes par pied au lieu d'un régime. Le cycle végétatif des bananiers plantains varie de 11 à 15 mois sauf pour la variété 'Apim' qui est de 18 mois. Des études de propagation rapide doivent être envisagées pour la sélection des variétés hautement performantes et la production massive du plantain pour les populations locales.

Optimized genetic transformation of Zanthoxylum zanthoxyloides by Agrobacterium rhizogenes and the production of chelerythrine and skimmiamine in hairy root cultures Zanthoxylum zanthoxyloides is an endangered African tree producing numerous bioactive substances including antileukemic and antisickling agents. Here, the potential of Z. zanthoxyloides hairy root cultures was tested for the production of bioactive substances with limited natural resources. The efficiency of Agrobacterium rhizogenes LBA9402-mediated transformation of leaf material was evaluated using different techniques. An optimal transformation frequency of 77% was obtained after 11 days by inoculating A. rhizogenes directly onto the central vein of 14-week-old leaves followed by a co-cultivation period of 3 days. Different treatments in immersion mode (manual wounding, acetosyringone, CaCl 2 , ultrasonication) never exceeded these results. A maximum growth rate of 0.37 cm/day was determined during the exponential phase. Liquid chromatography-diode array detection analysis showed the presence of skimmiamine, sesamine, chelerythrine, and chelerythrine derivatives in Z. zanthoxyloides hairy root lines. The maximum production of skimmiamine and chelerythrine in 28-day-old hairy root cultures was 45 ± 2 and 107 ± 4 mg/100 g dry weight, respectively. The present results highlight the potential of Z. zanthoxyloides hairy root cultures for the sustainable production of skimmiamine and chelerythrine.

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