Background: Some fungi have double host attacks for animals and plants, among them Alternaria alternata. Using a local medicinal plant such as Ephedra alanda is one way to fight its spreading. Aim: Evaluate the effect of the different extracts of Ephedra alanda against the mold Alternaria alternata. Material and Methods: This work used a different solvent by successive extraction of Ephedra alanda (Hexane, Chloroform, Ethyl acetate, and Methanol) and total alkaloids to evaluate the Anti- Alternaria alternata growth, besides assessing radical scavenging activity used the DPPH. Result: The result showed a meaningful reduction in the mold's diameter growth. All extracts of E. Alanda were effective against A. alternata growth, the concentration of inhibition varied between 5 mg/ml to 14 mg/ml; the total alkaloid was most efficient at a concentration of 0.12 mg/ml. The methanolic extract has an interesting antioxidant activity at 72% of inhibition. Conclusion: This study showed the ability of different extracts of Ephedra alanda (Hexane, Chloroform, Methanol and Alkaloids) to inhibit the growth of Alternaria alternata in the in-vitro culture.