Background Dental caries is a widespread disease that causes dental tissue destruction and leads to local and general complications. Gram-positive bacteria including Streptococcus mutans , Streptococcus sanguinis , and Enterococcus faecalis take part in dental caries formation. Gram-positive bacteria have cell walls that consistof a thick layer of peptidoglycan which maintains the strength and rigidity of the bacteria, as well as bacteria guard from internal osmotic pressure. The biosynthesis of peptidoglycan involves many enzymes, including the Mur family, penicillin binding protein (PBP), and sortases. Purpose This research has the intention to screen and examine the antibacterial compound of edible plant Kemangi ( Ocimum basilicum L.) in terms of how it fights against some oral pathogenic bacteria of E. faecalis ATCC 29212, S. mutans ATCC 25175, and S. sanguinis ATCC 10566. Materials and Methods The O. basilicum L. was macerated by several organic solvents to obtain the extracts, before then being purified using several combinations of chromatography methods and the compound was discovered via spectroscopic methods. For the assay against bacteria, the extracts and compounds were tested using agar well diffusion and microdilution assay. Results The isolated compound was identified as β-sitosterol. The compound activity against bacteria was evaluated by in vitro assay against S. sanguinis ATCC 10566 and E. faecalis ATCC 29212 with the MIC and MBC value of 25,000 and 50,000 ppm, respectively. The compound was also tested by in silico study using the molecular docking method. The molecular interaction between β-sitosterol and the protein target showed a lower binding affinity value than the native ligand and other positive controls for each protein. Based on the amino acid residue bound to the ligands, β-sitosterol on MurA and SrtA is not competitive to the positive control, showing potential as a natural antibacterial agent. Meanwhile, on the MurB and PBP, β-sitosterol and positive control do compete with each other. Conclusion The compound, isolated from O. basilicum L. leaf, was determined as β-sitosterol, which has the molecular formula C 29 H 50 O. The antibacterial activity of β-sitosterol by in vitro assay showed weak antibacterial activity, yet exhibited the potential to inhibit the biosynthesis of peptidoglycan and prevent bacteria cell wall formation by inhibiting MurA and SrtA activity via docking simulation.

Chitosan and gelatin were the most widely used natural materials in pharmaceutical and medical fields, especially as local hemostatic agents, independently or as a composite material with the addition of other active substances. Chitosan and gelatin have excellent properties in biocompatibility, biodegradability, non-toxicity and water absorption capacity. The objective of this review was to analyze the characteristics of chitosan-gelatin (CG) composite-based biomaterial and its effectivity as a local hemostatic agent. We used PRISMA guidelines and the PICO framework to compile this review. The findings demonstrated that the CG composite-based biomaterial had excellent physical, chemical, mechanical properties and local hemostatic agent activity by adding other active substances such as oxidized fibers (OF), silica nanoparticles (SiNPs), calcium (Ca) and biphasic calcium phosphate (BCP) or by setting the CG composite proportion ratio.

Background:Caries and periodontitis are dental diseases caused by bacteria of S. sanguinis, S. mutans, and E. faecalis with three main etiological factors of the host, substrate, and time. Objective:This study proposed to investigate the antibacterial effects of Buah Merah (Pandanus conoideus Lam.) against oral bacteria of E.faecalis, S. mutans, and S. sanguinis. Materials and Methods:The Buah Merah was extracted with different solvents to yield n-hexane, ethyl acetate, methanol, and H 2 O extracts. The concentrations of single and mixture extracts were adjusted for antibacterial assay against bacteria of E. faecalis, S. mutans, and S. sanguinis strains through agar well diffusion assay with chlorhexidine, fosfomycin, and quercetin used as positive controls. Results:The ethyl acetate extract showed highest antibacterial activity against three oral bacterial of E. faecalis, S. mutans, and S. sanguinis with inhibition zones values of 9.3, 12.3, and 17.9 mm at 40%, respectively, together with their MIC and MBC values of 1250 & 2500, 0.312 & 0.625, and 0.312 & 0.625 ppm, respectively. For the formulation of extracts, combinations samples test gave various effects to different bacteria, with the best activity showed by methanol-ethyl acetate (M-Ea) extracts against S. mutans with an inhibition zone of 16.25 mm at 40 ppm. The strong and synergistic effect of methanol extract against S. mutans was supported by inhibition zones of the formulation of methanol extract-fosfomycin which showed an inhibition zone of 25.9 mm at 10 ppm. Conclusion:The extracts of Buah Merah demonstrated antibacterial activity against oral bacteria of E. faecalis, S. mutans, and S. sanguinis and gave important information for further in vivo clinical studies to determine the exact dosages and its effectiveness in practical application. These results prove the antimicrobial effects of Buah Merah extracts as alternative natural drugs with synergistic effects of active constituents. of caries: host, substrate, and time. Streptococcus mutans is included as cariogenic bacteria because it is able to produce acid and carbohydrate quickly. The pathogenesis process of teeth caries is marked by the ability to grow in an acid surrounding and quick sugar metabolism characteristic for organic acid including lactic acid [1, 2].

Objective: A concern in fixed appliances orthodontic treatment besides reestablishing the normal occlusion characteristics might cause repositioning of the condyle position in TMJ region. The objective of this research is to find out whether there is a difference of TMJ position in Angle class I malocclusion type 1, 2, 3, 4, and 5 before and after fixed appliances orthodontic treatment. Methods:This research is a pre-and-post design study with analytical statistical approach which compared the TMJ position obtained from tracings 80 lateral cephalometric radiographs before and after fixed appliances orthodontic treatment. Purposive sampling technique was used to determine the sample size in Orthodontic Department of Rumah Sakit Gigi dan Mulut Universitas Padjadjaran from 2009-2015. The data was analyzed by using Kolmogorov-Smirnov Test and paired t-test.Results: This study revealed that the changes of condyle position in Condylion to Vertical T line (horizontally) after orthodontic treatment is not significant (P> 0.05) in Angle class I malocclusion. However, the difference of condyle position in Condylion to TC line (vertically) shows a significant difference (P< 0.05) in Angle class I malocclusion. Conclusion:The distance of the condyle from Condylion to TC line increased after fixed appliances orthodontic treatment for Angle class I malocclusion patients.

Introduction: Braces often used in the treatment of malocclusion. However, in recent years malocclusion no longer becomes the reason for orthodontic treatment. Many people use it merely for a lifestyle purpose, and most of them got it from the dental quack. This study was aimed to understand the influencing factors of the citizen of Bandung preferences towards dental quack on constructing custom-made orthodontic appliances. Methods: This study was a cross-sectional study with purposive sampling technique consisted of 30 samples. The inclusion criteria were subject wearing dental quack custom-made orthodontic appliance; citizen of Bandung; and willing to participate in the study. Exclusion criteria were subject refused to participate in the study. Results: The study showed that most of the subject (73%) were using the treatment for the teeth alignment, and only a few were using for the lifestyle purpose only. From all subjects wearing orthodontic appliance, 63% of them were chosen dental quack custom-made orthodontic appliance due to the lower price, and 37% of the rest due to peer’s suggestion who had it before. The combination of the low income with the needs of orthodontic treatment and inadequate information regarding orthodontic appliance such as dental braces affected the subjects preferences towards the dental quack custom-made orthodontic appliance. Overall, the socioeconomic environment is the most influencing factor of preference. Conclusion: A lower price was the most influencing factor of high preferences for the dental quack custom-made orthodontic appliance.