Silver nanoparticles were synthesized by green route method using green wheat spike extract. In the beginning the extract was prepared by boiling green wheat in water for 15 min and used for synthesis of silver nanoparticles. The starting material was silver nitrate solution (1mM) and green wheat extract was added at 90 C o for 30 min. The product is dark orange solution which is characterized by UV-vis spectrometry, where maximum absorption was 402 nm. FTIR analysis showed the absorption of active ingredients was adsorbed on the surface of silver nanoparticles. XRD pattern revealed the only phase of face centeredcubic presence as indication of silver me tal formation. FESEM image of silver nanoparticles that prepared by green wheat extract showed the spherical shape and their particles size was 20 nm in maximum and compatible with XRD calculations. The antibacterial activity was studied against Escherichia coli, Klebsiella pneumoniae, staphylococcus epidermidis, and streptococcus mutans