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An experiment was carried out at Navsari Agricultural University, Navsari during 2014 to evaluate the effect of pre-sowing treatments on survival percentage and growth of mango rootstocks. Mango stones were soaked in aqueous solutions of GA3 (100 and 200 ppm), Beejamruth (2 % and 3 %) and Thiourea (1 % and 2 %) for 12 and 24 hours. The trial was evaluated in Completely Randomized Design based on factorial concept and the treatments were replicated thrice. Imposition of treatments led to significant differences at 5 % level of significance for all parameters chosen in this study. Mango stones when treated with Thiourea at 1 % had the maximum shoot length (49.93 cm), root length (34.38 cm), shoot dry weight (21.08 g) and total dry weight (26.36 g). The highest number of lateral roots (10.90) and survival percentage (64.17) was observed in mango stones dipped in 100 ppm GA3. Between the two soaking duration, soaking mango stones for 24 hours recorded higher values for shoot length (45.03 cm), root length (32.79 cm), number of lateral roots (9.83), survival percentage (62.72), shoot root fresh weight ratio (4.30), shoot dry weight (21.33 g), total dry weight (26.28 g) and shoot root dry weight ratio (4.32). Thus, survival percentage and growth of mango rootstocks can be substantially improved by soaking mango stones in aqueous solutions of 100 ppm GA3 or Thiourea at 1 % for 24 hours before sowing.

The present investigation was carried out to assess the extent of genetic variability, heritability, genetic advance and genetic divergence using 33 gladiolus (Gladiolus grandiflorus Hort) genotypes with 16 characters. Moderate to high GCV and PCV were recorded for almost all the characters under study emphasizing the existence of variation in the population. All the characters (except number of days required for spike initiation, number of days required for first floret opening and number of florets/spike) showed high heritability along with higher/moderate genotypic coefficient of variation and genetic advance indicating that most likely the heritability was due to additive gene effects and the genotypes under study were highly diverse and of great potential with regard to these characters, and therefore, these are more reliable for effective phenotypic selection. The inter-cluster average D2 value was maximum (25.54) between cluster II and VI followed by between cluster V and cluster VI (24.36). The minimum inter cluster distance was obtained between cluster I and cluster III (12.34), indicated that genotypes of cluster I and cluster III are very close to each other. The clustering pattern showed that genotypes of different geographical areas were clubbed in one group and also the genotypes of same geographical area were grouped into same cluster as well as in different cluster indicating formal relationship between geographical diversity and genetic diversity.

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Three-dimensional (3D) imaging devices used for the visualization of anatomic structures not only allow for a more accurate diagnosis but also facilitate precise planning of surgical treatments, such as in guided surgery for instrument retrieval using templates. The innovative template-based treatment methods could also play a crucial role in future modern surgical endodontic treatments, enabling exact preplanning and precision-guided surgical interventions, and resulting in greater accuracy and success rates. We present here a case of a patient with a periapical lesion below a right mandibular second molar, with a fractured file segment extruding beyond the apex in the distal canal, and another instrument half-way extruded from the mesial canal, with radiolucencies evident at the mesial and distal root apices. The patient was treated by employing a 3D-guided microsurgical approach. First, 3D optical scans were imported into a guided surgery program. Periapical lesions and extruded fractured instruments were marked within the software. The osteotomy size, apical resection level, and bevel angle were defined pretreatment. This case introduces a novel-guided microsurgical endodontic technique, incorporating recommended guidelines of modern surgical endodontic treatment with the aid of 3D-printed surgical templates, by which minimally invasive surgical treatment was ensured with preservation of tooth, bone, and surrounding anatomical structures.