Petroleum ether extract of root part of cucumis prophetarum afforded one compound 4,4-dimethyl-Stigmast-5-22E,24-trien-3β-ol, which has structural similarity with Stigmast-5-22E-dien-24S-3β-ol and Propheterin. More over the 1 H-NMR and 13 C-NMR spectrum of the isolated compound matched with the literature values of previously reported data's whereas, the methanol extract afforded one compound 7,8,11,12,14,15,16,17-octahydro-17-((E)-6-hydroxy-6-methylhept-3-en-2-yl)-9,13dimethyl-6H-cyclopenta[a]phenanthren-7-yl acetate. The structural elucidation of these compounds was conducted using 1 H-NMR, 13 C-NMR, UV, IR and DEPT spectroscopic methods.