Objective To assess prevalence of intestinal parasite and associated factors among patients with human immune deficiency virus (HIV)/ acquired immune deficiency syndrome (AIDS) in Debre markos referral hospital, Amhara region, Ethiopia from 2015-2019RESULTS This study revealed that, out of 380 patients, the prevalence of intestinal parasites was found to be 24.2% with95%CI (18.9, 28.4). The most predominant parasite was both forms of Entamoeba histolytica 11.3% followed by G.lambilia 8.9%, Strongyloides sterocoralis 2.4%, but A.lumbricoid 0.8% and H.worm 0.8% were the least detected. ART status had significant association with prevalence of intestinal parasites (AOR =3.566, CI: 1.882, 6.758).