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This work describes the characterization of the grafted 2-benzo[c]cinnoline (2BCC) molecules at a glassy carbon (GC) electrode surface by voltammetry and spectroscopy. Attachment of the molecule to the carbon substrate was achieved by the electrochemical reduction of 2-benzo[c]cinnoline diazonium salt (2BCC-DAS). GC electrode modification was carried out in aprotic solution with 2BCC diazonium salt. Dopamine (DA) and ascorbic acid (AA) were used to prove the surface modification to see the blockage of the electron transfer. The presence of 2BCC at the GC electrode surface was characterized by cyclic voltammetry and Raman spectroscopy. Raman spectroscopy was used to monitor molecular bound properties of the adsorbates at the 2BCC-GC surface and confirm the attachment of 2BCC molecules onto the GC surface. The thickness of the 2BCC film on GC was also investigated by ellipsometric measurement.

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