Aim. To study the activity of myeloperoxidase in the peripheral blood of pregnant women in normal and thyroid diseases. Materials and methods. Cytochemical analysis was used to study the activity of myeloperoxidase in peripheral blood neutrophils of pregnant women with diffuse toxic goiter, as well as in women with the physiological course of pregnancy in a comparative aspect. Results. It was found that in women with a physiological course of pregnancy, the cytochemical parameters of myeloperoxidase in neutrophils were reduced only in the 1st trimester of pregnancy. In patients with diffuse toxic goiter, with severe thyrotoxicosis syndrome, the activity of the enzyme was significantly reduced during all periods of pregnancy. Apparently, these shifts of the enzyme, which is the main component of the protective system of blood phagocytes, are associated with their functional stress. Conclusion. Based on this opinion, it seems that blood neutrophils enter the system of thyroid hormone metabolism by their degradation. Apparently, this mechanism is activated with an increased content of hormones in the circulation, which occurs during pregnancy, and especially with concomitant diffuse toxic goiter with thyrotoxicosis syndrome and is aimed at preserving homeostasis necessary for normal fetal development.

Purpose: The aim of this study was to determine the possibility of PG90 using as an indicator of PD and reveal the optimal cut-off point for separation of normal glucose metabolism (NGM) and impaired (PD+DM) glucose metabolism (IGM) by PG90 level. Methods: 134 persons (32 men and 102 women) aged 20-79 years were examined. Glycemic levels after 30, 60, 90,120 minutes after 75 g glucose loading was performed. The HbA1C examined by the SDA1c Care (SD biosensor, Korea). Fasting and post load venous plasma glucose was determined by Precision PCx Medi Sense (Abbot, USA). Equations obtained Bby linear and multiple regression method; by applying these equations 90 minutes glycemic level post-load 75 g glucose was calculated. Qualitative characteristics of the diagnostic test were used for the evaluation of cut-off point for 90 minutes glycemic level post-load 75 g glucose. Results: The relationship between PG90 and HbA1C, PG90 and FG, PG90 and PG120 was studied by using correlation analyses. The relationship between PG90 and HbA1C (r=+0.71 [95%CI +0.615, +0.785]), PG90 and FG (r=+0.53 [95%CI +0.397, +0.641]) was as well as between PG90 and PG120 (r=+0.85 [95%CI +0.796, +0.891]). All three correlation coefficients were statistically significant, p<0.001. The equation for PG90 cut-off point was calculated by applying multiple regression analyses. It was obtained the quality characteristics of received cut-off point by equation. Maximal Index Youden had FG cut-off point “>109 mg/dl” (67.9%). Index Youden for PG90 cut-off point “>168 mg/dl” was higher than 50.0 %. Conclusion: The cut-off point PG90 may be used for NGM and PD differentiation