Cone yoghurt is a yoghurt variety produced by adding only pine cones to milk without culture in a limited area of Turkey. The present study was conducted to identify and characterize lactic flora in traditional cone yoghurt. Morphological, cultural, physiological, biochemical, and genotypic characteristics were employed to identify lactic acid bacteria isolates from cones and cone yoghurts. subsp. () and subsp. () were obtained from both cones and yoghurts. Among the isolates, was frequently isolated except for these two bacteria ( and ). The results indicate that the cone yoghurt has a mixed microflora contrary to the yoghurt produced by the addition of a starter culture and, and in cone yoghurt originates from the pine cones.

The purpose of this study were to identify the presence of E.coli O157 and to determine its prevalence in foods which were collected from various restaurants, shops and markets in Istanbul. Also, validation of detection method of E. coli O157 in all food stuffs was carried out according to applicability, repeatability, and minimum detection limit (LOD) and false positive and negative analysis based on TS EN ISO 16654 standard method. The results showed that the prevalence of E. coli O157 in food was 2%, and its prevalence increased in April and May.

A: Investigation of Rotavirus, Adenovirus and Astrovirus in mussels and shrimps using multiplex real-time PCR. Kafkas Univ Vet Fak Derg, 24 (5): 761-767, 2018761-767, . DOI: 10.9775/kvfd.2018 Abstract Viruses are one of the most common pathogens transmitted via food. Based on epidemiological evidence, human enteric viruses are considered to be as the most commonly transmitted etiological agents by bivalve shellfish. In Istanbul, the most widely consumed sea products after fish are mussels and shrimps. In this study, the presence of rotavirus, adenovirus and astrovirus were investigated in mussel and shrimp samples consumed by hunting on the shores of Istanbul. For this purpose, a total of 28 groups of shrimp and 52 groups of mussel were collected from different places in Istanbul. Each group was analyzed separately as a sample. Viruses were extracted from the digestive tissue by direct elusion method in a glycine/NaCl, pH 9.5 buffer followed by PEG-6000 precipitation. Multiplex Real-time RT-PCR technique was used to analyze the shellfish samples. Astrovirus was found in 63.46% of the total 52 analyzed mussel samples, whereas adenovirus was found in 46.15%. None of the mussel samples was positive for rotavirus. All tested shrimp samples were negative for all three researched viruses. This is the first report on the prevalence of rotavirus, adenovirus and astrovirus in mussels and shrimps in Turkey. The results show that mussels sold in Istanbul are highly contaminated with adenovirus and astrovirus and this could pose a great threat to public health. Keywords: Mussel, Shrimp, Rotavirus, Adenovirus, Astrovirus, RT-PCR Midye ve Karideslerde Rotavirus, Adenovirus ve Astrovirus Varlığının Multiplex Real-time PCR Kullanılarak Araştırılması ÖzViruslar gıda vasıtasıyla en yaygın bulaşan patojenlerden biridir. Epidemiyolojik bulgulara göre insana ait enterik viruslar kabuklu deniz ürünleri vasıtasıyla en çok aktarılan etiyolojik ajan olarak tanımlanmaktadır. İstanbul'da balıktan sonra en çok tüketilen deniz ürünleri midye ve karidestir. Bu çalışmada, İstanbul kıyılarında avlanarak tüketilen midye ve karides numunelerinde rotavirus, adenovirus ve astrovirus varlığı araştırılmıştır. Bu amaçla toplam 28 karides ve 52 midye grubu İstanbul'un farklı yerlerinden toplanmıştır. Her bir grup ayrı bir numune olarak analize alınmıştır. Viruslar glisin/NaCl, pH 9.5 tamponu kullanarak direkt elüsyon metodu ile doğrudan sindirim dokusundan ayrıştırılmış ve PEG-6000 ile konsantre edilmiştir. Alınan midye ve karides numuneleri Multiplex Real-time PCR yöntemi ile analiz edilmiştir. Toplam çalışılan 52 midye numunesinin %46.15'inde adenovirus ve %63.46'sında astrovirus saptanmıştır. Rotavirus hiçbir midye numunesinde tespit edilmemiştir. Karides numunelerinin hiçbirinde araştırılan virusların hiçbiri saptanmamıştır. Bu, Türkiye'deki midyelerde ve karideslerde rotavirus, adenovirus ve astrovirus prevalansı hakkındaki ilk rapordur. Sonuçlar, İstanbul'da satılan midyelerin adenovirus ve astrovirus ile kontamine olduğunu ve bu durumun halk s...

In parallel with the world population growth, the decrease in food sources, caused by global climate change, unplanned urbanization, unplanned industrialization and reduction of agricultural land etc., creates a high potentially risk about poor nutrition and hunger. This case has caused scientists to make researches about new food sources and alternative nutrients. Numerous processes and materials such as algae, edible insects, microbial proteins, microbial oils, in vitro meat, non-dairy and vegan milk and cheese, bio-fermentation technology have been proposed as alternatives by scientists. Besides being healthy of these foods, characteristics such as price, taste, shelf life will be decisive for their acceptance thereof by consumers. However, cultural, religious and social factors may be limiting on the alternative foods. Despite all, future generations will probably eat very different foods for nutrition that we consume today. In this study, it is aimed to give information about possible future alternative food sources and technologies, mainly on protein sources.

Bruselloz büyük ölçüde ekonomik kayıplara yol açabilen, aynı zamanda halk sağlığı ve gıda güvenliği açısından da oldukça tehlikeli bir hastalıktır. Bu hastalığın önemi göz önünde bulundurulduğunda, süt ve süt ürünlerinde tanımlanması hayati önem taşımaktadır. Bu çalışmada İstanbul / Beylikdüzü semtinde açıkta satışa sunulan toplamda 100 adet süt ve süt ürünü (25 adet çiğ süt, 45 adet peynir, 15 adet tereyağ ve 15 adet kaymak) Brucella spp. varlığı yönünden araştırılmıştır. Örneklerden Brucella spp. izolasyon ve identifikasyonu için, kültür, biyokimyasal testler, süt ring testi, aglütinasyon reaksiyonu testleri kullanılmıştır. Süt ring testinde 4 adet pozitif sonuç elde edilmiş ancak bunlarda Brucella spp. izole edilememiştir. Yüz adet numuneden izole edilen şüpheli kolonilerden biyokimyasal testler ve aglütinasyon testleriyle son olarak beş adedi belirlenmiş, ancak İstanbul Pendik Veteriner